1IRN

RUBREDOXIN (ZN-SUBSTITUTED) AT 1.2 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1993-06
Detector	MARRESEARCH
Wavelength(s)	0.93
Spacegroup name	H 3
Unit cell lengths	64.100, 64.100, 33.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.200
R-factor	0.1068
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.200 *
Rmerge	0.059	0.260 *
Number of reflections	15659 *
Completeness [%]	99.0 *
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4	23 *	pH 4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.8-1.6 (mM)
2	1	drop	ammonium sulfate	0.82 (M)	20%saturated
3	1	drop	citrate	30 (mM)
4	1	reservoir	ammonium sulfate	2.46 (M)	60%saturated
5	1	reservoir	citrate	100 (mM)