1IRJ
Crystal Structure of the MRP14 complexed with CHAPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH AND SE-MAD WAVELENGTHS |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.70000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.590, 178.440, 61.230 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.2449 * |
| Rwork | 0.245 |
| R-free | 0.27360 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.990 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 * | 0.339 * |
| Total number of observations | 251869 * | |
| Number of reflections | 65554 | 9583 * |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Itou, H., (2001) Acta Crystallogr, D57, 1174. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.8-8.2 |
| 5 | 1 | reservoir | PEG6000 | 1-2 (%(w/v)) | |
| 6 | 1 | reservoir | CHAPS | 0.25-1.75 (%(w/v)) |
