1IQY
CRYSTAL STRUCTURE OF NICKEL-SUBSTITUTED AMINE OXIDASE FROM ARTHROBACTER GLOBIFORMIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.800, 62.840, 184.070 |
| Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOLO-AGAO |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.038 | |
| Total number of observations | 364483 * | |
| Number of reflections | 146419 | |
| Completeness [%] | 94.8 | 81.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 16 * | potassium-sodium tartrate, pH 6.8, MICRODIALYSIS, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | potassium sodium tartrate tetrahydrate | 1.05 (M) | |
| 3 | 1 | 2 | HEPES | 25 (mM) | pH6.8 |
