1IQX

CRYSTAL STRUCTURE OF COBALT-SUBSTITUTED AMINE OXIDASE FROM ARTHROBACTER GLOBIFORMIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-06-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	I 1 2 1
Unit cell lengths	157.990, 62.770, 184.190
Unit cell angles	90.00, 111.85, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.213
Rwork	0.211
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOLO-AGAO
RMSD bond length	0.010
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.074
Total number of observations	480123 *
Number of reflections	155223 *
Completeness [%]	99.8	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.8	16 *	potassium-sodium tartrate, pH 6.8, MICRODIALYSIS, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	potassium sodium tartrate tetrahydrate	1.05 (M)
3	1	2	HEPES	25 (mM)	pH6.8