1IQP
Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-07-08 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.361, 105.608, 316.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.037 | 0.143 |
| Total number of observations | 260398 * | |
| Number of reflections | 76556 | |
| <I/σ(I)> | 30 | 4.5 |
| Completeness [%] | 93.5 | 78.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 293 | MPD, glycerol, sodium chloride, acetic acid, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 10 | 1 | drop | acetate | 100 (mM) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | 450 (mM) | ||
| 4 | 1 | drop | glycerol | 6 (%) | |
| 5 | 1 | drop | BES | 4 (mM) | |
| 6 | 1 | drop | 0.03 (mM) | ||
| 7 | 1 | reservoir | acetate | 100 (mM) | |
| 8 | 1 | reservoir | MPD | 42.5-47.5 (%(v/v)) | |
| 9 | 1 | reservoir | glycerol | 5-7.5 (%(v/v)) |
