1IQ8

Crystal Structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-20
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	99.282, 99.282, 363.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.227 *
Rwork	0.227
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.006 *
RMSD bond angle	1.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.092	0.256
Total number of observations	530165 *
Number of reflections	88967 *	4241 *
<I/σ(I)>	34	7.1
Completeness [%]	95.3	93
Redundancy	6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ishitani, R., (2001) Acta Cryst., D57, 1659. *