1IQ4

5S-RRNA BINDING RIBOSOMAL PROTEIN L5 FROM BACILLUS STEAROTHERMOPHILUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-08
Detector	MARRESEARCH
Wavelength(s)	0.7
Spacegroup name	C 1 2 1
Unit cell lengths	138.650, 49.220, 68.930
Unit cell angles	90.00, 117.32, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.215 *
Rwork	0.215
R-free	0.25910
Structure solution method	MAD and MR
RMSD bond length	0.045
RMSD bond angle	21.880 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.239 *
Total number of observations	252800 *
Number of reflections	37507
<I/σ(I)>	7.6	3.7
Completeness [%]	97.7	96.6
Redundancy	6.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	18 *	PEG8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	PEG8000	16 (%)
4	1	reservoir	MPD	10 (%)