1IQ1

CRYSTAL STRUCTURE OF THE IMPORTIN-ALPHA(44-54)-IMPORTIN-ALPHA(70-529) COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	78.939, 89.623, 100.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.209 *
Rwork	0.209
R-free	0.24800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ial
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.124	0.561
Total number of observations	142209 *
Number of reflections	18165 *
<I/σ(I)>	8.9	1.3
Completeness [%]	97.6 *	96.9
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	291	Fontes, M.R.M., (2000) J. Mol. Biol., 297, 1183. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium citrate	0.55 (M)
2	1	1	HEPES	100 (mM)
3	1	1	dithiothreitol	10 (mM)