1IPW

INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI WITH THREE MAGNESIUM IONS

Experimental procedure

Source type	SEALED TUBE
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	H 3 2
Unit cell lengths	109.400, 109.400, 154.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.171
Rwork	0.171
R-free	0.23900
RMSD bond length	0.010
RMSD bond angle	24.400 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.048
Number of reflections	13745
Completeness [%]	79.7	67.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium citrate	0.8 (M)
2	1	1	HEPES	100 (mM)