1IPI
CRYSTAL STRUCTURE OF THE ARCHAEAL HOLLIDAY JUNCTION RESOLVASE HJC FROM PYROCOCCUS FURIOSUS FORM II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-11 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 0.834 |
| Spacegroup name | P 65 |
| Unit cell lengths | 59.969, 59.969, 134.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.000 - 2.160 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1GEF functional dimer |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.846 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 * | 2.260 |
| High resolution limit [Å] | 2.160 * | 2.160 |
| Rmerge | 0.064 * | 0.279 |
| Number of reflections | 13043 | |
| <I/σ(I)> | 6.42 | 2.7 |
| Completeness [%] | 88.9 | 79.5 |
| Redundancy | 7.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 20 * | 100mM Tris-HCl, 1mM EDTA, 32.5% PEG4000, 10% glycerol , pH 7.0, VAPOR DIFFUSION, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 100 (mM) | pH7.0 |
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 1 | PEG4000 | 32.5 (%(w/v)) | |
| 5 | 1 | 1 | glycerol | 10 (%) |
