1IPI

CRYSTAL STRUCTURE OF THE ARCHAEAL HOLLIDAY JUNCTION RESOLVASE HJC FROM PYROCOCCUS FURIOSUS FORM II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-11
Detector	RIGAKU RAXIS
Wavelength(s)	0.834
Spacegroup name	P 65
Unit cell lengths	59.969, 59.969, 134.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.000 - 2.160
R-factor	0.214 *
Rwork	0.214
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1GEF functional dimer
RMSD bond length	0.006
RMSD bond angle	23.846 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000 *	2.260
High resolution limit [Å]	2.160 *	2.160
Rmerge	0.064 *	0.279
Number of reflections	13043
<I/σ(I)>	6.42	2.7
Completeness [%]	88.9	79.5
Redundancy	7.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	20 *	100mM Tris-HCl, 1mM EDTA, 32.5% PEG4000, 10% glycerol , pH 7.0, VAPOR DIFFUSION, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris-HCl	100 (mM)	pH7.0
3	1	1	EDTA	1 (mM)
4	1	1	PEG4000	32.5 (%(w/v))
5	1	1	glycerol	10 (%)