1IPH

STRUCTURE OF CATALASE HPII FROM ESCHERICHIA COLI

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	95.200, 134.700, 124.400
Unit cell angles	90.00, 109.40, 90.00

Refinement procedure

Resolution	8.000 * - 2.800
R-factor	0.2
Rwork	0.200
RMSD bond length	0.019
RMSD bond angle	3.490
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.108 *
Total number of observations	65329 *
Number of reflections	225245 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	15 (%(w/v))
2	1	reservoir		1.5 (M)
3	1	reservoir	Tris-HCl	0.2 (M)