1IPD

THREE-DIMENSIONAL STRUCTURE OF A HIGHLY THERMOSTABLE ENZYME, 3-ISOPROPYLMALATE DEHYDROGENASE OF THERMUS THERMOPHILUS AT 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	78.600, 78.600, 158.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 2.200
R-factor	0.185
RMSD bond length	0.015
RMSD bond angle	0.036 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.042 *
Total number of observations	42499 *
Number of reflections	24478 *
Completeness [%]	83.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *		taken from Karsube, Y. et al (1988). J. Biochem., 104, 679-680. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	28 (mg/ml)
2	1	drop	phosphate	10 (mM)
3	1	drop	ammonium sulfate	0.66 (M)	precipitant