1IPA

CRYSTAL STRUCTURE OF RNA 2'-O RIBOSE METHYLTRANSFERASE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.97951, 0.979242, 0.982049, 0.975887
Spacegroup name	P 43 21 2
Unit cell lengths	66.729, 66.729, 125.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 2.400
R-factor	0.226 *
Rwork	0.226
R-free	0.28600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.051	0.204
Total number of observations	130341 *
Number of reflections	12905 *
<I/σ(I)>	10.7	3.3
Completeness [%]	98.3	98.6
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.7M ammonium sulfate, 1.7% 2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.7M ammonium sulfate, 1.7% 2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	dioxane	6.0 (%)