1IP4

G72A HUMAN LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-01-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.467, 61.622, 32.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.171
Rwork	0.171
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800
Rmerge	0.029
Total number of observations	55173 *
Number of reflections	10477
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	10 *	sodium phosphate, sodium chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		2 (M)
2	1	reservoir	sodium acetate	0.02 (M)