1IOT

STABILIZATION OF HEN EGG WHITE LYSOZYME BY A CAVITY-FILLING MUTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	296
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.160, 79.160, 37.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.750
R-factor	0.171
RMSD bond length	0.009
RMSD bond angle	23.107 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.760
Rmerge	0.058
Number of reflections	10397
Completeness [%]	88.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	296	NaCl, Na-acetete, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 296K