1IOP
INCORPORATION OF A HEMIN WITH THE SHORTEST ACID SIDE-CHAINS INTO MYOGLOBIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.020, 91.020, 45.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SPERM WHALE MYOGLOBIN |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.645 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.145 |
| Total number of observations | 185449 * | |
| Number of reflections | 17073 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 96.3 | 94.4 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 11 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | potassium cyanide | 10 (mM) | |
| 4 | 1 | 2 | ammonium sulfate | 3.7 (M) |
