1IOD

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE COAGULATION FACTOR X BINDING PROTEIN FROM SNAKE VENOM AND THE GLA DOMAIN OF FACTOR X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Collection date	1997-05-11
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	99.760, 99.760, 90.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.201
Rwork	0.201
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.330
Total number of observations	109232 *
Number of reflections	20411	3206 *
<I/σ(I)>	18.3
Completeness [%]	96.4	92.5 *
Redundancy	4.9	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.9	293	PEG8000, calcium chloride, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	10 (mM)
2	1	reservoir	PEG8000	12 (%)
3	1	reservoir		5 (mM)