1IOA
ARCELIN-5, A LECTIN-LIKE DEFENSE PROTEIN FROM PHASEOLUS VULGARIS
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-02-22 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.300, 94.500, 82.900 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26900 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 28.220 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.085 |
| Number of reflections | 16994 |
| Completeness [%] | 97.3 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 20 (%(w/v)) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | sodium acetate | ||
| 5 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | sodium acetate |
