1IO0
CRYSTAL STRUCTURE OF TROPOMODULIN C-TERMINAL HALF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7 |
| Spacegroup name | H 3 |
| Unit cell lengths | 69.294, 69.294, 101.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.450 |
| Rwork | 0.203 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.073 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.076 | 0.286 |
| Total number of observations | 357913 * | |
| Number of reflections | 31954 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 22 * | PEG 400, zinc sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | glycine | 25 (mM) | pH3.0 |
| 3 | 1 | drop | MES-NaOH | 0.1 (M) | pH6.5 |
| 4 | 1 | drop | PEG400 | 24 (%(v/v)) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | reservoir | MES- NaOH | 0.1 (M) | pH6.5 |
| 7 | 1 | reservoir | PEG400 | 15 (%(v/v)) | |
| 8 | 1 | reservoir | 6 (mM) |
