1INP
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 1-PHOSPHATASE AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 41 |
| Unit cell lengths | 51.640, 51.640, 143.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Total number of observations | 63687 * |
| Number of reflections | 17652 |
| Completeness [%] | 99.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | bovine 1-ptase | 13 (mg/ml) | |
| 2 | 1 | drop | bis-Tris | 10 (mM) | |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | PEG8000 | 13 (%) | |
| 5 | 1 | drop | 200 (mM) | ||
| 6 | 1 | drop | Tris | 100 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 13 (%) | |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | Tris | 100 (mM) |
