1IMJ
CRYSTAL STRUCTURE OF THE HUMAN CCG1/TAFII250-INTERACTING FACTOR B (CIB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.704, 44.445, 43.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.200 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.23500 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.300 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.039 | 0.105 |
| Total number of observations | 47199 * | |
| Number of reflections | 10809 | |
| Completeness [%] | 94.0 | 88.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 * | Padmanabhan, B., (2000) Acta Crystallogr, D56, 1479. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 278 * | Padmanabhan, B., (2000) Acta Crystallogr, D56, 1479. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.55 (M) | |
| 6 | 1 | reservoir | urea | 12 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 80 (mM) | pH7.5 |
| 8 | 1 | reservoir | glycerol | 5 (%) |
