1IM9
Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1 bound to its MHC ligand HLA-Cw4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.631, 75.867, 125.746 |
| Unit cell angles | 90.00, 120.96, 90.00 |
Refinement procedure
| Resolution | 29.210 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QQD PDB entry 1NKR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 | 0.191 |
| Number of reflections | 41881 | 3503 * |
| <I/σ(I)> | 10.2 | 3 |
| Completeness [%] | 97.9 | 82.3 |
| Redundancy | 4. * | 2. * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | PEG6000, magnesium chloride, ethylene glycol, N-(2-acetamindo)iminodiacetic acid , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 5 (%) | |
| 2 | 1 | reservoir | 80 (mM) | ||
| 3 | 1 | reservoir | glycerol | 6 (%) | |
| 4 | 1 | reservoir | ADA | 50 (mM) | |
| 5 | 1 | drop | protein | 12 (mg/ml) |
