1IM2

HslU, Haemophilus Influenzae, Selenomethionine Variant

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.925, 0.979, 1.068
Spacegroup name	P 6 2 2
Unit cell lengths	109.826, 109.826, 313.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.900 - 2.800
R-factor	0.233
Rwork	0.233
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g41
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.112	0.305
Total number of observations	190830 *
Number of reflections	43125
<I/σ(I)>	8.3	4.8
Completeness [%]	90.5	61.3
Redundancy	7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	291	PEG 2000 MME, lithium sulphate, magnesium sulphate, zink sulphate, tricine, europium chloride, ADP, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		0.1 (M)
10	1	reservoir		1 (mM)
11	1	reservoir		3 (mM)
12	1	reservoir	tricine	100 (mM)
2	1	drop	beta-mercaptoethanol	5 (mM)
3	1	drop		1 (mM)
4	1	drop	potassium phosphate	20 (mM)
5	1	drop	ADP	10 (mM)
6	1	reservoir	PEG2000 MME	10 (%)
7	1	reservoir		0.6 (M)
8	1	reservoir	ethanol	6 (%)
9	1	reservoir		10 (mM)