1ILV
Crystal Structure Analysis of the TM107
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-23 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9793,0.9791,0.9639 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.522, 115.522, 78.666 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.560 - 2.000 |
| R-factor | 0.244 * |
| Rwork | 0.244 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| Starting model (for MR) | wARP autotraced model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.126 | 0.614 |
| Total number of observations | 328134 * | |
| Number of reflections | 79278 * | |
| <I/σ(I)> | 19.3 | 2.5 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | ammonium sulphate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.75 (M) |
