1ILT
X-RAY STRUCTURE OF INTERLEUKIN-1 RECEPTOR ANTAGONIST AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.110, 71.110, 112.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.069 * |
| Completeness [%] | 95.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 20 * | drop contained 1:1 mixture of precipitant and recombinant IL-lra * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | IL-lra | 21 (mg/ml) | |
| 2 | 1 | drop | MOPS | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 30 (%) | precipitant |
| 4 | 1 | reservoir | 0.6 (M) | precipitant | |
| 5 | 1 | reservoir | MES | 50 (mM) | precipitant |
