1IJX

CRYSTAL STRUCTURE OF THE CYSTEINE-RICH DOMAIN OF SECRETED FRIZZLED-RELATED PROTEIN 3 (SFRP-3;FZB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9203
Spacegroup name	P 1 21 1
Unit cell lengths	96.050, 48.690, 83.310
Unit cell angles	90.00, 90.80, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.22 *
Rwork	0.220
R-free	0.25700
Structure solution method	MAD
RMSD bond length	0.009 *
RMSD bond angle	1.480 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.077	0.679
Number of reflections	61070
<I/σ(I)>	26.3	2.2
Completeness [%]	99.8	98.7
Redundancy	7.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	20 *	PEG 3350, PEG 400, Sodium Hepes, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	PEG3350	12 (%)