1IJX
CRYSTAL STRUCTURE OF THE CYSTEINE-RICH DOMAIN OF SECRETED FRIZZLED-RELATED PROTEIN 3 (SFRP-3;FZB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9203 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.050, 48.690, 83.310 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.480 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.077 | 0.679 |
| Number of reflections | 61070 | |
| <I/σ(I)> | 26.3 | 2.2 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 20 * | PEG 3350, PEG 400, Sodium Hepes, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | PEG3350 | 12 (%) |
