1IJT

Crystal Structure of Fibroblast Growth Factor 4 (FGF4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.97940
Spacegroup name	P 21 21 21
Unit cell lengths	40.367, 53.295, 56.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
R-factor	0.194 *
Rwork	0.194
R-free	0.20670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fgf
RMSD bond length	0.005
RMSD bond angle	1.313
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.109
Total number of observations	40562 *
Number of reflections	11590 *
<I/σ(I)>	16.5
Completeness [%]	99.6	99.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	PEG 8000, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	drop	HEPES-NaOH	25 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG8000	30 (%)
5	1	reservoir	ammonium sulfate	0.2 (M)