1IJQ

Crystal Structure of the LDL Receptor YWTD-EGF Domain Pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	108
Detector technology	CCD
Collection date	2000-01-28
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	76.284, 76.284, 265.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.208
Rwork	0.208
R-free	0.25000
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.560
High resolution limit [Å]	1.500	1.500
Rmerge	0.069	0.100
Total number of observations	543455 *
Number of reflections	127540
<I/σ(I)>	22.7
Completeness [%]	88.3	55.8
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG4000,2-propanol,HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG4000,2-propanol,HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	reservoir	2-propanol	10 (%(v/v))
4	1	reservoir	PEG4000	20 (%(w/v))