1IJH
CHOLESTEROL OXIDASE FROM STREPTOMYCES ASN485LEU MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 86 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-09 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.261, 72.881, 63.034 |
Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.530 |
R-factor | 0.163 * |
Rwork | 0.163 |
R-free | 0.21270 |
Structure solution method | OTHER |
Starting model (for MR) | 1b4v |
RMSD bond length | 0.009 |
RMSD bond angle | 0.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.580 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.045 | 0.265 |
Number of reflections | 63695 | |
<I/σ(I)> | 25.5 | 4.6 |
Completeness [%] | 92.8 | 73.8 |
Redundancy | 4.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 17 * | Yue, K., (1999) Biochemistry, 38, 4277. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.5 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | reservoir | PEG8000 | 10-12 (%(w/v)) | |
4 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
5 | 1 | reservoir | 75 (mM) |