1IJ5
METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-01-01 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.400, 64.400, 207.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| Rwork | 0.244 |
| R-free | 0.26000 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 3.100 |
| High resolution limit [Å] | 3.000 | 2.990 |
| Rmerge | 0.064 | 0.337 |
| Total number of observations | 38752 * | |
| Number of reflections | 9795 * | |
| Completeness [%] | 91.7 | 96.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 25 * | Iwasaki, W., (1999) J.Biochem., 126, 7. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 25 * | Iwasaki, W., (1999) J.Biochem., 126, 7. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 74 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.6 |
| 3 | 1 | reservoir | ammonium sulfate | 1.4-1.8 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | EDTA | 10 (mM) | |
| 6 | 1 | reservoir | 0.02 (%) | ||
| 7 | 1 | reservoir | HEPES-NaOH | 0.1 (M) | pH6.4-7.6 |
