1IJ1

GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	25.683, 42.192, 42.682
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	20.000 - 1.860
R-factor	0.227
Rwork	0.227
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.940
High resolution limit [Å]	1.860 *	1.860
Rmerge	0.046	0.306
Total number of observations	80296 *
Number of reflections	8284
<I/σ(I)>	12.4	4.5
Completeness [%]	97.6	94.4 *
Redundancy	4.6	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sparse matrix method *	6	298	0.1 M Na Cacodylate, 20% PEG 8000, 0.2 M Zn Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium cacodylate	0.1 (M)
2	1	1	zinc acetate	0.2 (M)
3	1	1	PEG8000	20 (%)