1IJ0

Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 1 21 1
Unit cell lengths	35.466, 28.616, 44.496
Unit cell angles	90.00, 109.60, 90.00

Refinement procedure

Resolution	20.000 - 1.860
R-factor	0.211 *
Rwork	0.211
R-free	0.25800
RMSD bond length	0.013
RMSD bond angle	1.900
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.940
High resolution limit [Å]	1.860	1.860
Rmerge	0.065	0.032 *
Total number of observations	70770 *
Number of reflections	6740
<I/σ(I)>	15.4
Completeness [%]	97.6	93.7 *
Redundancy	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sparse matrix method *	7	297	0.1 M Na Cacodylate, 16% PEG 8000, 0.1 M Zn Acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium cacodylate	0.1 (M)
2	1	1	zinc acetate	0.2 (M)
3	1	1	PEG8000	16 (%)