1IJ0
Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.466, 28.616, 44.496 |
Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.860 |
R-factor | 0.211 * |
Rwork | 0.211 |
R-free | 0.25800 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.900 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.065 | 0.032 * |
Total number of observations | 70770 * | |
Number of reflections | 6740 | |
<I/σ(I)> | 15.4 | |
Completeness [%] | 97.6 | 93.7 * |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sparse matrix method * | 7 | 297 | 0.1 M Na Cacodylate, 16% PEG 8000, 0.1 M Zn Acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium cacodylate | 0.1 (M) | |
2 | 1 | 1 | zinc acetate | 0.2 (M) | |
3 | 1 | 1 | PEG8000 | 16 (%) |