1IIW
GLUR0 LIGAND BINDING CORE: CLOSED-CLEFT LIGAND-FREE STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.258, 49.483, 56.374 |
| Unit cell angles | 90.00, 117.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.204 |
| Total number of observations | 110565 * | |
| Number of reflections | 18470 | |
| <I/σ(I)> | 18 | 4.4 |
| Completeness [%] | 97.5 | 81.1 |
| Redundancy | 3.01 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 4 * | 34% MPD, 100 mM Na Acetate, 5% glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 34-38 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | glycerol | 5 (%) | |
| 5 | 1 | drop | glycerol | 5 (%) |
