1IIT

GLUR0 LIGAND BINDING CORE COMPLEX WITH L-SERINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	CCD
Collection date	2000-06-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	97.865, 49.769, 55.633
Unit cell angles	90.00, 117.23, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.209 *
Rwork	0.209
R-free	0.25000
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.094
Total number of observations	131611 *
Number of reflections	18810
<I/σ(I)>	27.2	19.3
Completeness [%]	99.9	100
Redundancy	7	6.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	4 *	38% MPD, 0.2 M LiCl, 0.1 M Na Acetate, 10 mM L-Serine, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	MPD	34-38 (%)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir		0.2 (M)
5	1	drop	L-serine	10 (mM)