1IIT
GLUR0 LIGAND BINDING CORE COMPLEX WITH L-SERINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-06-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.865, 49.769, 55.633 |
| Unit cell angles | 90.00, 117.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.25000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.094 |
| Total number of observations | 131611 * | |
| Number of reflections | 18810 | |
| <I/σ(I)> | 27.2 | 19.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 4 * | 38% MPD, 0.2 M LiCl, 0.1 M Na Acetate, 10 mM L-Serine, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 34-38 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | drop | L-serine | 10 (mM) |
