1IIR
Crystal Structure of UDP-glucosyltransferase GtfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-08 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.110, 102.110, 83.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.23100 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 | 0.281 |
| Number of reflections | 41091 | |
| <I/σ(I)> | 26.8 | 5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | magnesium sulfate, PEG 400, MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 1.53 (M) | ||
| 3 | 1 | reservoir | PEG400 | 2 (%) | |
| 4 | 1 | reservoir | MES | 100 (mM) |
