1II6

Crystal Structure of the Mitotic Kinesin Eg5 in Complex with Mg-ADP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-16
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	53.083, 78.588, 94.147
Unit cell angles	90.00, 93.84, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.2178 *
Rwork	0.218
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	catalytic core of KAR3
RMSD bond length	0.012 *
RMSD bond angle	0.467 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.125	0.103
Total number of observations	236822 *
Number of reflections	42896
<I/σ(I)>	9.4
Completeness [%]	94.9	85
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8 *	4 *	used seeding *
1	VAPOR DIFFUSION, SITTING DROP	6.8 *	4 *	used seeding *
1	VAPOR DIFFUSION, SITTING DROP	6.8 *	4 *	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG3350	18 (%)
3	1	reservoir	PIPES	100 (mM)
4	1	reservoir		200 (mM)