1II4

CRYSTAL STRUCTURE OF SER252TRP APERT MUTANT FGF RECEPTOR 2 (FGFR2) IN COMPLEX WITH FGF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-26
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	70.970, 72.657, 89.739
Unit cell angles	89.98, 89.70, 90.00

Refinement procedure

Resolution	25.000 - 2.700
R-factor	0.238
Rwork	0.238
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ev2
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.050	0.250
Total number of observations	74426 *
Number of reflections	46770 *
<I/σ(I)>	12.1
Completeness [%]	91.6	79.7
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	298	PEG 4000, Isopropanol, HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES-NaOH	25 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG4000	10-15 (%)
5	1	reservoir	isopropanol alcohol	10 (%)
6	1	reservoir	HEPES-NaOH	0.1 (M)