1IHJ
Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0072 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.170, 44.150, 44.460 |
| Unit cell angles | 106.55, 100.61, 118.25 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.2198 |
| Rwork | 0.220 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BE9 minus peptide |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.306 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.059 | 0.156 |
| Number of reflections | 25906 | 2424 * |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 95.1 | 86.7 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 24 (%) | |
| 3 | 1 | reservoir | Tris | 100 (mM) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | glycerol | 10 (%) |
