1IHA

Structure of the Hybrid RNA/DNA R-GCUUCGGC-D[BR]U in Presence of RH(NH3)6+++

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-03-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	53.800, 19.400, 50.310
Unit cell angles	90.00, 109.90, 90.00

Refinement procedure

Resolution	1.750 - 1.600
R-factor	0.161
Rwork	0.162
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	165D from pdb
RMSD bond length	0.011
RMSD bond angle	0.035
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.500	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.141
Total number of observations	40122 *
Number of reflections	6312
<I/σ(I)>	21	3.8
Completeness [%]	96.0 *	89
Redundancy	6.2 *	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	MPD, Rhodium hexammine, lithium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	Rhodium hexammine
3	1	1	lithium cacodylate
4	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	lithium cacodylate	0.05 (M)
2	1	drop	RNA/DNA hybrid		0.1mg
3	1	drop	rhodium/iridium hexammine		0.1mg
4	1	drop		5 (%)
5	1	reservoir	MPD	35-40 (%)