1IGM

THREE DIMENSIONAL STRUCTURE OF AN FV FROM A HUMAN IGM IMMUNOGLOBULIN

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	43.700, 43.700, 212.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.300
R-factor	0.201
RMSD bond length	0.013
RMSD bond angle	0.029
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.070 *
Total number of observations	30357 *
Number of reflections	8539 *
Completeness [%]	84.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	20-22 *	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	80 (mg/ml)
2	1	reservoir	PEG600	24-25 (%(v/v))
3	1	reservoir	Tes	0.02 (M)