Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-10 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 33.730, 33.730, 129.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.28500 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 21.600 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.700 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.075 | 0.210 * |
| Total number of observations | 54446 * | |
| Number of reflections | 11090 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 81.0 | 58 |
| Redundancy | 4.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.4 | 298 | 3M ammonium sulphate, 100 mM magnesium chloride, pH 6.4, EVAPORATION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 75 (%) | |
| 3 | 1 | drop | magnesium chloride | 40-120 (mM) |
