1IF7
Carbonic Anhydrase II Complexed With (R)-N-(3-Indol-1-yl-2-methyl-propyl)-4-sulfamoyl-benzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-25 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.800, 41.800, 73.400 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.980 |
| Rwork | 0.214 |
| R-free | 0.29400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2cba |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.980 |
| Rmerge | 0.098 |
| Total number of observations | 119392 * |
| Number of reflections | 21083 * |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 96.0 * |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | methyl mercuric acetate | 1 (mM) | |
| 3 | 1 | drop | Tris-sulfate | 20 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.75 (M) | precipitant |
| 5 | 1 | reservoir | Tris-sulfate | 50 (mM) | precipitant |
