1IF6
Carbonic Anhydrase II Complexed With 3,5-difluorobenzenesulfonamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-17 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.600, 41.900, 73.500 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.090 |
| Rwork | 0.211 |
| R-free | 0.29900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2cba |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.090 |
| Rmerge | 0.090 |
| Number of reflections | 13211 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 95.1 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | methyl mercuric acetate, tris-sulfate, ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
