1IES

TETRAGONAL CRYSTAL STRUCTURE OF NATIVE HORSE SPLEEN FERRITIN

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-06-01
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	147.229, 147.229, 152.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.201
Rwork	0.201
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hrs
RMSD bond length	0.012
RMSD bond angle	2.400
Data reduction software	XDS
Data scaling software	MARSCALE
Phasing software	AMoRE
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.700
High resolution limit [Å]	2.500	2.500
Rmerge	0.118	0.365
Total number of observations	354156 *
Number of reflections	51340
<I/σ(I)>	19.8	1.8
Completeness [%]	89.5	60.8
Redundancy	6.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	0.66 (M)
2	1	reservoir	cadmium sulfate	80 (mM)
3	1	reservoir	sodium azide	3 (mM)