1IER

CUBIC CRYSTAL STRUCTURE OF NATIVE HORSE SPLEEN FERRITIN

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1993-02-01
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	182.900, 182.900, 182.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.260
R-factor	0.186 *
Rwork	0.187
Starting model (for MR)	1hrs
RMSD bond length	0.017
RMSD bond angle	20.600 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.380
High resolution limit [Å]	2.250 *	2.260
Rmerge	0.110 *
Total number of observations	88762 *
Number of reflections	12699
<I/σ(I)>	6.5	1.8
Completeness [%]	99.3	99.7
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.0 (M)
2	1	reservoir	cadmium sulfate	80 (mM)
3	1	reservoir	sodium azide	3 (mM)