1IE8
Crystal Structure Of The Nuclear Receptor For Vitamin D Ligand Binding Domain Bound to KH1060
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.490, 51.870, 131.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.520 |
R-factor | 0.212 * |
Rwork | 0.212 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.560 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.048 | 0.290 |
Number of reflections | 45925 | |
<I/σ(I)> | 20.4 | 4.6 |
Completeness [%] | 98.1 | 97.3 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES/KOH | 50 (mM) | |
3 | 1 | drop | Tris-HCl | 5 (mM) | |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | ammonium sulfate | 0.7 (M) | |
6 | 1 | reservoir | MES- KOH | 100 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 1.4 (M) |