1IE0
CRYSTAL STRUCTURE OF LUXS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.732, 62.732, 150.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20900 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.817 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.063 | 0.357 |
| Total number of observations | 413208 * | |
| Number of reflections | 23431 | |
| <I/σ(I)> | 30.5 | |
| Completeness [%] | 98.0 | 92.8 |
| Redundancy | 8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | Ammonium sulfate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | tri(carboxyethyl)phosphine | 2 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 5 | 1 | reservoir | Tris | 100 (mM) |
