1IDO

I-DOMAIN FROM INTEGRIN CR3, MG2+ BOUND

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.9300, 0.9686, 0.9793, 0.9797
Spacegroup name	P 43
Unit cell lengths	45.700, 45.700, 94.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.700
R-factor	0.202
Rwork	0.202
Structure solution method	MAD SOFTWARE USED : MLPHARE STARTING MODEL FOR MOLECULAR REPLACEMENT: NULL
RMSD bond length	0.012
RMSD bond angle	24.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	1.780
High resolution limit [Å]	1.700	1.710
Rmerge	0.046	0.250
Number of reflections	21824
Completeness [%]	96.8	98.6
Redundancy	2.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	PEG8000	12 (%)
3	1	reservoir	Tris	0.1 (M)
4	1	reservoir		1 (mM)
5	1	reservoir	beta-mercaptoethanol	10 (mM)