1IDN

MAC-1 I DOMAIN METAL FREE

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1994-08
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	49.320, 124.050, 76.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.174
Rwork	0.174
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	0.037
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.940
High resolution limit [Å]	2.700	2.700
Rmerge	0.097 *	0.173 *
Number of reflections	9011
<I/σ(I)>	16.3	4.6
Completeness [%]	68.0	39.8
Redundancy	5.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5		reservoir was mixed 1:1 with 3 microlitter protein *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop	sodium azide	0.025 (%)
4	1	reservoir	PEG6000	20-24 (%)
5	1	reservoir	Na acetate	100 (mM)