1ICS
CRYSTAL STRUCTURE OF 12-OXOPHYTODIENOATE REDUCTASE 1 FROM TOMATO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 1 |
Unit cell lengths | 53.857, 72.027, 72.357 |
Unit cell angles | 62.92, 85.17, 78.69 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.197 * |
Rwork | 0.197 |
R-free | 0.23700 |
Starting model (for MR) | pdb: 1ICP |
RMSD bond length | 0.008 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.038 | 0.136 |
Total number of observations | 73998 * | |
Number of reflections | 40681 | |
<I/σ(I)> | 17.1 | 4 |
Completeness [%] | 96.6 | 89.6 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 18 * | Tris/HCl, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MOPS-KOH | 10 (mM) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.45 (M) | |
5 | 1 | reservoir | PEG400 | 2 (%) |